ActionStep
In the Process Picture:4
•
Click on the buffer inlets.
•
Select the position of one of the inlets that will be used at the beginning of the run.
Result: The inlet valve switches to the selected port.
In the Process Picture:5
•
Click on the Pumps.
•
Set the System flow to 1.0 ml/min for ÄKTA pure 25 or 10.0 ml/min for ÄKTA pure 150.
•
Click Set flow rate.
Result: A system flow starts.
Connect a 25 to 30 ml syringe to the purge valve of the left pump head of System pump
B. Make sure that the syringe fits tightly into the purge connector.
6
Open the purge valve by turning it counter-clockwise about three quarters of a turn.
Draw a small volume of liquid slowly into the syringe (with a rate of about 1 ml per second).
7
Close the purge valve by turning it clockwise. Disconnect the syringe and discard its
contents.
8
ÄKTA pure User Manual 29119969 AB 165
5 Operation
5.4 Prime inlets and purge pump heads
5.4.1 System pumps
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