ActionStep
Connect the syringe to the purge valve on the right pump head of System pump B, and
repeat steps 6 to 8. Keep the system flow running.
9
Validate purge of pump B
Follow the instructions below to check that there is no air left in the pump after performing a purge.
ActionStep
In the Process Picture:1
•
Click on the Injection valve and select Manual load.
Result: The Injection valve switches to manual load position.
Make sure that the pump flow is on.2
In the Chromatogram pane:
Check the PreC pressure curve.
3
If the PreC pressure do not stabilize within a few minutes there may be air left in the
pump. Refer to Section 8.6 Troubleshooting: Pumps, on page 387.
166 ÄKTA pure User Manual 29119969 AB
5 Operation
5.4 Prime inlets and purge pump heads
5.4.1 System pumps