Purge the Sample pump
Follow the instruction below to purge both the pump heads of the Sample pump.
ActionStep
Make sure that all sample inlet tubing that is to be used during the method run is immersed
in the correct buffers.
1
Make sure that the waste tubing connected to Injection valve port W2 is immersed in a
waste vessel.
2
Open the System Control module.3
In the Process Picture :4
•
Click on Injection valve and select Sample pump waste .
Result: The injection valve switches to waste position. This is necessary to achieve a
low back pressure during the purge procedure.
In the Process Picture: Click on Sample pump.5
•
If Sample inlet valve V9-IS or V9H-IS is used: Click on the Sample inlet valve icon (if
the valve is not used, the icon will not be present):
Select the Buffer inlet.
•
Click on the Sample pump icon: Set the Sample flow to 1.0 ml/min for ÄKTA pure 25
or 10.0 ml/min for ÄKTA pure 150.
•
Click and close the pop-up window.
Result: a sample pump flow starts.
170 ÄKTA pure User Manual 29119969 AB
5 Operation
5.4 Prime inlets and purge pump heads
5.4.2 Sample pump
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