Shimadzu RF-20A - 7.5 Wavelength Calibration Operation Procedure

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5. Movement of the emission-side grating to the 254 nm position
The excitation-side grating is aligned with the 254 nm position determined in 7. Next, the
emission-side grating is gradually rotated from near 250 nm slightly below 254 nm for the low-
pressure Hg (Mercury) lamp, and the light intensity peak is recorded by the emission-side light
sensor. The wavelength value of this maximum value is obtained by using the span correction
coefficient (S value) calculated in 19, and is recorded as 254 nm on the emission side.
6. Movement of the emission-side grating to the 507 nm position
The emission-side grating is gradually rotated from near 500 nm slightly below 507 nm for the
low-pressure Hg (Mercury) lamp, and the light intensity peak is recorded by the emission-side
light sensor. The wavelength value of this maximum value is obtained by using the span
correction coefficient (S value) calculated in 19, and is recorded as 507 nm on the emission side.
7. Movement of the emission-side grating to the 761 nm position
The emission-side grating is gradually rotated from near 755 nm slightly below 761 nm for the
low-pressure Hg (Mercury) lamp, and the light intensity peak is recorded by the emission-side
light sensor. The wavelength value of this maximum value is obtained by using the span
correction coefficient (S value) calculated in 19, and is recorded as 761 nm on the emission side.
8. Restoring the grating position
The excitation-side and emission-side gratings are returned to their positions before wavelength
calibration was started.
No
EX
EM
1
HP Peak detection
HP Peak detection
2
254nm Scan
254nm
3
507nm Scan
254nm
4
761nm Scan
254nm
5
254nm
254nm Scan
6
254nm
507nm Scan
7
254nm
761nm Scan
8
Measurement result
7.4.1 Judgment of Wavelength Accuracy and Display of Results
Judgment of wavelength accuracy
Control criteria: 253.7 ±2.0 nm, 507.3 ±2.0 nm, 761.0 ±2.0 nm
7.5 Wavelength Calibration Operation Procedure
(1) Wavelength calibration can be executed only in the 1-wavelength mode. Set the
measurement mode to the 1-wavelength mode.
(2) In the case of the RF-20A, turn OFF and remove the Xenon lamp, and attach the low-
pressure Hg (Mercury) lamp.
(3) Using the pump or syringe, deliver or fill the flow cell with one of water, methanol or
acetonitrile.
(4) Make sure that there are no air bubbles in the flow cell, and attach the flow cell onto the
instrument.
(5) Execute "FACTORY" - "WAVE ADJ".
(6) Enter "CELL No.".
(7) Wavelength calibration is started.
(8) When wavelength calibration ends, the wavelength accuracy check is started automatically,
and the results are displayed on screen.