Shimadzu RF-20A - Compensating Sensitivity (Eliminating Physical Differences between Instruments); 8.1 Compensating Sensitivity (SENS COMP); 8.2 Adjustment using peak height of the Raman spectrum of water; 8.3 Compensating and Verifying Sensitivity at the Factory (Reference)

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8 Compensating Sensitivity (Eliminating Physical
Differences between Instruments)
8.1 Compensating Sensitivity (SENS COMP)
Sensitivity is adjusted before shipment from the factory by quinine sulfate solution. However, this
sensitivity compensation method is not used in the field since it is difficult to obtain quinine sulfate
solution. Compensate sensitivity by the following method when physical differences between
instruments appear after having replaced optical elements or having adjusted the optical axis.
 Analyze the customer's sample, and enter a compensation coefficient within the range 0.1 to
10 (default: 1) at "SENS COMP" in the calibration support group. The -HV of SENSE 1 to 3 is
automatically compensated.
 Before shipment from the factory, the height (F value) of the peak of the Raman spectrum
of water is verified. However, this peak contains lots of unstable factors which makes it
unsuitable for use in the field. JAIMAS either does not require verification using the peak
height of water Raman spectrum. Therefore, verification using the water Raman spectrum
is abolished in RF-20A validation. During the S/N CHECK, use the displayed F value just
as reference.
8.2 Adjustment using peak height of the Raman spectrum of water
It adjusts by Raman spectrum of water.Procedure is written to Chapter 15.6 “Compensating
Sensitivity After Photomultiplier Replacement (adjustment using peak height of the Raman
spectrum of water)AAA)”.
8.3 Compensating and Verifying Sensitivity at the Factory (Reference)
The procedure for compensating sensitivity before shipment from the factory is described below
for reference only.
8.3.1 Adjusting and Verifying Sensitivity
8.3.2 Jig Flow Line Diagram
LC pump: LC-20AD 3 units
Resistor tube: SUS316 I.D. 0.1  4 m, 3 sets
High-pressure valve: FCV-20AH2 2 units (FCV-12AH 2 units + sub-controller)
Quinine sulfate/0.1N sulfuric acid: Preparation and storage are compliant with 228-34706.
0.1 N sulfuric acid
Drain
Drain
Drain
Drain
Stop plug
Stop plug
RF-20A(xs)
LC pump 3
Resistor tube 3 sets
High-pressure valve 2 pc
DGU-20A3
0.005 μg/mL
0.1 μg/mL
Quinine sulfate/0.1 N
sulfuric acid
Chromatopac
ETFE i. d. 0.3
SUS or PEEK i.d. 0.3
PEEK i.d. 0.3
Quinine sulfate/0.1 N
sulfuric acid