Using the Chiller
Operation 6-3
calibration wavelengths are good enough to approximate the dispersion for the
intermediate wavelengths.
The operator can now select and display the new calibrated objective. The dispersion for
a particular experimental setup can now be automatically compensated for according to
the objective table when the wavelength is changed, thus providing the shortest pulses
possible (and the brightest multi-photon fluorescence signal) for any wavelength in the
calibration range.
If conditions change during the experiment (e.g., the imaging depth changes), the
operator can adjust the motor position slightly (using the Motor
Position control; see
Figure 6-9) during the experiment to modify the dispersion from the calibrated value.
As mentioned earlier, motor positions for non-calibrated wavelengths are calculated
from the adjacent calibration points on either side of the current wavelength using linear
interpolation. If a calibration point is missing from one side, the motor position value is
set to be equal to the existing adjacent calibration wavelength.
Using the Chiller
Refer to the chiller user’s manual for information on how to operate that unit.
In general, the reservoir should always be full before turning on the unit, and the chiller
should be set to 21°
C whenever the laser is running.
It takes the chiller about 15 minutes to stabilize at the coolant temperature of 21°C. Due
to its mass, it takes the laser housing longer to reach steady state temperature. Leaving
the chiller on between periods of laser use eliminates this stabilization period. In
general, if the laser is used often, leave the chiller on between laser usage. If the laser is
used infrequently, turning the chiller and power supply off saves energy, but a longer
warm up time should be expected.
The chiller must be on whenever the power supply is on!
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