Measure Protein Bradford
Thermo Scientific NanoDrop One User Guide 107
Protein Bradford standard curve screen
The standard curve screen shows graphically the relationship between the measured standards,
the calculated standard curve, and the measured absorbance and calculated concentration for
a selected sample. A horizontal line connects the sample absorbance value on the Y-axis to the
standard curve. A vertical line connects that point to the sample concentration value on the
X-axis.
The R
2
value indicates how well the standard curve fits the standard data points (1.0 is a
perfect fit; that is, all points lie exactly on the curve).
Note
• A baseline correction is performed at 750 nm (absorbance value at 750 nm is
subtracted from absorbance values at all wavelengths in sample spectrum).
• Micro-volume absorbance measurements and measurements taken with nonstandard
cuvettes are normalized to a 10.0 mm pathlength equivalent.
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