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NanoDrop One Viewer Software
Thermo Scientific NanoDrop One User Guide 273
Automated pathlength Affects micro-volume measurements only.
• When Automated Pathlength is selected, software selects the optimal pathlength
(between 1.0 mm and 0.03 mm) based on sample absorbance at the analysis
wavelength. For example, when sample absorbance at the analysis wavelength is
less than or equal to 12.5 (10 mm pathlength equivalent), the optimal longer
pathlength is used. When sample absorbance is greater than 12.5, the optimal
shorter pathlength is used. Recommended for samples that are highly absorbing at
the analysis wavelength. (This option may cause reduced sensitivity when the
sample spectra have a large absorbance peak that is not at the analysis
wavelength.)
Note: When the analysis wavelength is between 190 nm and 219 nm, the optimal
longer pathlength is used when sample absorbance is less than or equal to 10
(10 mm pathlength equivalent), and the optimal shorter pathlength is used when
sample absorbance is greater than 10.
• When Automated Pathlength is deselected, the software uses a 1 mm pathlength
regardless of the sample absorbance. This can cause detector saturation (resulting
in jagged peaks) for highly absorbing samples (e.g., ~15 A at 10 mm pathlength
equivalent).
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