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Learning Center
NanoDrop One Viewer Software
272 NanoDrop One User Guide Thermo Scientific
• Select curve fit type.
Specify type of equation used to create standard curve from standard concentration
values. Available options:
– Linear: Draws the linear least squares line through all measured standards
(requires reference measurement and at least one standard)
– Interpolation: Draws a series of straight lines to connect all measured
standards (requires reference measurement and at least one standard)
– 2
nd
order polynomial: Draws the 2
nd
order least squares polynomial using
all measured standards (requires reference measurement and at least two
standards)
– 3
rd
order polynomial: Draws the 3
rd
order least squares polynomial using all
measured standards (requires reference measurement and at least three
standards)
Baseline correction Select this option to correct offset caused by light scattering particulates by subtracting
the absorbance at a specified baseline point. Then specify wavelength for baseline
correction.
Note: Software subtracts absorbance value at specified baseline correction wavelength
from absorbance values at all wavelengths in sample spectrum. As a result, absorbance
of sample spectrum is zero at specified baseline correction wavelength.
Analysis wavelength
correction
Use this option to specify absorbance correction at analysis wavelength only. Available
options:
• None. No correction at analysis wavelength.
• Single point. Enter wavelength for analysis correction. (Absorbance value at
specified analysis correction wavelength is subtracted from absorbance value at
analysis wavelength. Corrected value is used to calculate sample concentration.)
• Sloping baseline. Enter two wavelengths that define sloping baseline for analysis
correction. (Absorbance value of sloping baseline at analysis wavelength is
subtracted from absorbance value at analysis wavelength. Corrected value is used
to calculate sample concentration.)
Setting Available Options
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