8 Maintaining the COC Inlet
To Install a Capillary Column with the Cool On-Column Inlet
Maintaining Your GC 125
6 Break off the column end by supporting it against the column cutter opposite the scribe.
Inspect the end with a magnifying loupe to make certain there are no burrs or jagged
edges.
7 Wipe the column walls with a tissue dampened with isopropanol to remove fingerprints
and dust.
8 Gently insert the column into the inlet until it bottoms. You should feel spring tension as
you push up on the column. (Do not withdraw the column.)
9 Insert the column nut into the inlet fitting and finger-tighten.
To avoid bending the inlet, always use two wrenches. Use a 5/16-inch wrench to support the
inlet while tightening the column nut with a 1/4-inch wrench.
10 Tighten the column nut an additional 1/4 turn with a wrench or until the column does not
move.
11 If using an automatic injection system with a 0.25-mm or 0.32-mm column, verify that the
column installation by manually pushing the syringe into the inlet.