BD LSR II - Page 202

202 BD LSR II User’s Guide
pulse
definition 136
measurements 137
processors 137
Q
quality control (QC)
about 49
log 141
particles 152
troubleshooting 123
R
Radius laser 33
recording
calcium flux data 98
compensation Tubes 63, 64
data 66, 68
optimization 88
red laser 33
removing air bubbles, filter 47, 48
replacing
Bal seal 112
battery 108
optical filters 42
sample tube O-ring 114
sheath filter 110, 111
requirements specifications xiii
reusing analyses 74
running samples 51
S
safety
biological xx
electrical xix
general xxi
laser xvii
symbols and labels xxi
sample
injection tube 30
optimization 53
experiment 57
single-stained controls 54, 66
tube requirements 116
SAMPLE FINE ADJ knob 28
sample injection port (SIP) 126
cleaning 102
components 29, 30
problems with 116
replacing Bal seal 112
replacing sample tube O-ring 114
samples, running 68
Sapphire laser 33
saving analyses 74
scatter
light 127
parameter distortion 121
setting laser delay 195
setup, instrument 53
sheath
clamp knob 44
container 31, 44
filter, replacing 110, 111
flow 126
fluid 126
backflush 30
pressure 126
shortpass (SP) filters 34, 131
Show Population Hierarchy 70
shutdown, instrument 102
side scatter (SSC) 33, 127
signals, amplifying 35
singlet population, discrimination 84
SIP See sample injection port.
software
adjusting detector voltages 36
instrument control xi
LSR2.book Page 202 Tuesday, April 25, 2006 3:34 PM