411829-L, Operation April 2012 Page 4-12
4
after. If no peak of interest has been set, the load capacity for each cartridge type is calculated
according to the smallest CV.
12. To close the TLC to Step Gradient editor and open a method containing the purification gradient
at the Method tab, press OK.
13. Set up the rest of the method parameters; see “Specify the Method Parameters at the
Parameters Tab” on page 4-4 and “Specify Collection Parameters at the Collection Tab” on
page 4-8.
14. To save the method and reuse it in the future, see “Save and/or Run the Method” on page 4-9.
15. To run a purification using the (saved or unsaved) method, see “Prepare and Run a Purification”
on page 4-15.
Figure 4-7. TLC to Step Gradient Editor
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