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11.2 Getting the Print Head Ready for Printing
Fill the biggest (10 mL) syringe barrel [1] with the material that you want to use as the core material.
Insert a piston to assure an even distribution of the air pressure. Attach the 10 mL syringe barrel to
a Male to Female Luer Coupling [2] and twist the syringe barrel & coupling into the middle inlet stem
of the needle [3]. The male end connects to the needle, while the female end connects to the syringe.
Use a ¼” wrench to keep the needle stem stationary while twisting the Luer coupling in its place.
Next, fill the right-hand 5 mL (from the front) syringe barrel [4] with the material intended to be the
middlemost material and insert a piston. Attach a Female Luer to Barb fitting [5] to the syringe barrel.
Connect the other end of the fitting to the Tygon tubing [6] and connect the other end of the tubing
to the upper needle inlet stem [7].
Repeat the same process with the left-hand 5 mL syringe barrel [8], which will contain the material
used as an outer shell material. Connect the left syringe barrel with the assistance of the Female
Luer to Barb fitting [5] to the lower needle inlet stem [9]. Insert the two side barrels into the print
head by pushing them into the barrel holder until they lock into their place.
Also, if working with a photosensitive bioink, keep the bioink protected from light by using orange
UV-protected syringe barrels to avoid premature crosslinking. Turn off the Brinter
®
casing lights to
work in a dark mode.
Attach the pressure hoses by twisting the pressure connectors [10] to the syringe barrels. Make sure
that the pressure hose is tightly attached to the pressure connector on the print head.
Before printing, open the pressure valves [11] for the channels that are intended to be used for the
print job. The valve is open when the knob is aligned to the direction of the tubing. Adjust the printing
pressure for the main syringe barrel (10 mL) from the software UI. Adjust the pressure for the two
side barrels by pushing and turning the air pressure regulator knobs [12] in the clockwise direction.