H50/H51 Hematology Analyzer User Manual Measurement Principle
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Chapter 3 Measurement Principle
3.1 WBC, RBC and PLT Measurement Principle
In accordance with electrical impedance method based on Coulter Principle, WBC total counts is
measured in the WBC counting chamber , RBC total counts and PLT total counts in the RBC
counting chamber. This method is based on the modification of the impedance of a calibrated
aperture soaking in an electrolyte and going through a constant current delivered by two
electrodes located on both sides of the aperture. A vacuum applied on a side of the aperture
allows the cells passage. Cells oppose their physical volume to the current passage. A voltage
impulse is registered at the electrodes terminal. The height of this impulse is proportional to the
cell volume.
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