H50/H51 Hematology Analyzer User Manual Measurement Principle
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3.2 Five-part Diff Measurement Principle
The principle of this technology is based on the introduction of the sample solution and a
sheathing liquid (Diluent) at the same in the flow cell. Thus, the sheathing liquid drives the
sample flow straight across the cuvette through the detection area.
The main advantages of the measurement principle are:
1) High level of reliability of the optical adjustment
2) Only two measurement axes for five parameters
3) High resolution matrix
4) Low level of contamination between two measurements
5) Low Reagents consumption
WBC 5 differential percentages are obtained by optic measurements. The measured pulses on the
two optical channels are displayed on DIF Plot ALL (Y axis) and FSC (X axis). Each dot on the
DIF Plot represents the height in ALL and FSC of at least one pulse.
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