H50/H51 Hematology Analyzer User Manual Measurement Principle
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3.3 HGB Measurement Principle
3.4 MCV and MPV Measurement
MCV is the average volume of individual erythrocytes. Based on RBC histogram, the analyzer
measure MCV directly.
MPV is the average volume of individual platelets. Based on PLT histogram, the analyzer
measure MPV directly.
3.5 Equations for Calculated Parameters
3.5.1 Calculation of WBC-Related Parameters
1) Lymphocyte absolute value (LYM#) is made from LYM% and WBC total counts by formula
below:
LYM# = LYM % WBC (total counts)
2) Monocyte absolute value (MON#) is made from MON% and WBC total counts by formula
below:
MON# = MON% WBC (total counts)
3) Neutrophil absolute value (NEU#) is made from NEU% and WBC total counts by formula
below:
NEU# = NEU% WBC (total counts)
4) Eosinophil absolute value (EOS#) is made from EOS% and WBC total counts by formula
below:
EOS# = EOS% WBC (total counts)
5) Basophils absolute value (BAS#) is made from BAS% and WBC total counts by formula
below:
BAS# = BAS% WBC (total counts)
6) ALY% is the percentage of the LYM based on a fixed threshold in the lymphocyte’s
population area
The HGB measurement is directly done in the WBC
chamber, by spectrophotometer (545 nm). H
GB
is
detected by formation of oxyhemoglobin.
HGB blank check is performed at each STARTUP
cycle. HGB Blank measurement
is done also at the
beginning of each analysis cycle.
By comparison of
the two values, it is possible to follow potential
evolution of the blank value in order to warn the user
if necessary.
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