H50/H51 Hematology Analyzer User Manual Measurement Principle
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7) Abnormal lymphocyte absolute value (ALY#) is made from ALY% and lymphocyte absolute
value by formula below:
ALY# = ALY% x LYM#
8) IMM% is the number of elements in the IMM population area divided by sum of elements
in DIF scattergram including IMM
9) Immature monocyte absolute value (IMM#) is made from IMM% and WBC total counts by
formula below:
IMM# = IMM% x WBC (total counts)
3.5.2 Calculation of RBC-Related Parameters
1) Hematocrit (HCT) is measured by integration of the volume of the red blood cells which
flow in the RBC counting chamber aperture.
HCT=
ୖେ୶େ
ଵ
Where HCT is expressed in %, RBC is expressed in 10
12
/L and MCV is expressed in fL.
ʹሻ Mean Corpuscular Hemoglobin (MCH) calculation is obtained from HGB and RBC by the
following formula
MCH =
ୌୋ
ୖେ
Where MCH is expressed in pg, HGB is expressed in g/L and RBC is expressed in 10
12
/L.
3) Mean Corpuscular Hemoglobin Concentration (MCHC) is made from HGB and HCT by the
formula below:
MCHC =
ୌୋ୶ଵ
ୌେ
Where MCHC is expressed in g/L, HGB is expressed in g/L and HCT is expressed in %.
4) RDW CV parameter is derived from the RBC histogram.
5) RDW SD parameter is calculated on curve distribution (defined as curve width at 20% of
peak)
3.5.3 Calculation of PLT-Related Parameters
1) Thrombocrit (PCT) is made from PLT and MPV by formula below:
PCT =
୶
ଵ
Where PCT is expressed in %, PLT is expressed in 10
9
/L and MPV is expressed in fL.
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