Chapter 4 Microscopy Techniques
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Objective
DF microscopy requires objectives (excluding 2x and 4x) with NA smaller than the minimum NA of the
condenser. Using an objective with a diaphragm, the diaphragm in the objective can be adjusted, enabling
DF microscopy.
TI-DF dark-field condenser adapter
Use the TI-DF dark-field condenser adapter to mount a darkfield condenser lens.
TI-DF dark-field condenser adapter
Condenser lens
Condenser lenses that allow DF microscopy are as follows:
Usable condenser lens
Darkfield condenser (dry type) Darkfield condenser (oil type)
NA 0.80 to 0.95 1.2 to 1.43
Working
distance
4 mm 1.5 mm
Microscopy
technique
DF DF
Othe
The TI-DF dark-field condenser adapter is required.
T-CHNA High N.A. common condenser lens unit (optional)
Use the T-CHNA High N.A. common condenser lens unit to increase the brightness during DF microscopy.
T-CHNA High N.A. common condenser lens unit
Condenser adapter provided with the T-CHNA High N.A. common condenser lens unit
The T-CHNA High N.A. common condenser lens unit is equipped with a dedicated condenser adapter.
When using a T-CHNA High N.A. common condenser lens unit, use the provided condenser adapter to
mount the condenser lens (TI-DF is unnecessary).
Darkfield Condenser Dry Darkfield Condenser Oil
High NA lens Condenser adapter