Nikon ECLIPSE Ti2-U - Page 6

Contents
iv
3.15 Using a Centering C-mount Adapter ............................................................................................ 78
Chapter 4 Microscopy Techniques ............................................................................................................ 79
4.1 Details of Diascopic Bright-field (BF) Microscopy ........................................................................ 80
4.1.1 Principles of BF Microscopy ........................................................................................... 80
4.1.2 Optical Elements Required for BF Microscopy ............................................................... 81
4.1.3 Combination of Optical Elements ................................................................................... 82
4.2 Details of Phase Contrast (Ph) Microscopy ................................................................................. 83
4.2.1 Principles of Ph Microscopy ............................................................................................ 83
4.2.2 Optical Elements Required for Ph Microscopy ............................................................... 84
4.2.3 Combination of Optical Elements ................................................................................... 86
4.3 Details of Diascopic DIC and IMSI Microscopies ......................................................................... 87
4.3.1 Principles of DIC Microscopy .......................................................................................... 87
4.3.2 Optical Elements Required for DIC Microscopy ............................................................. 88
4.3.3 Combination of Optical Elements ................................................................................... 90
4.4 Details of Episcopic Fluorescence (Epi-FL) Microscopy .............................................................. 94
4.4.1 Principles of Epi-FL Microscopy...................................................................................... 94
4.4.2 Optical Elements Required for Epi-FL Microscopy ......................................................... 95
4.4.3 Combination of Optical Elements ................................................................................... 96
4.5 Details of Diascopic Dark-field (DF) Microscopy .......................................................................... 97
4.5.1 Principles of DF Microscopy ........................................................................................... 97
4.5.2 Optical Elements Required for DF Microscopy ............................................................... 97
4.5.3 Combination of the Optical Elements Required for DF Microscopy ............................... 99
4.6 Details of NAMC Microscopy ..................................................................................................... 100
4.6.1 Principles of NAMC Microscopy ................................................................................... 100
4.6.2 Optical Elements Required for NAMC Microscopy ....................................................... 101
4.6.3 Combination of Optical Elements ................................................................................. 102
Chapter 5 Troubleshooting ....................................................................................................................... 103
5.1 Troubleshooting for Microscopy Techniques .............................................................................. 104
5.1.1 Common Microscopy Troubles ..................................................................................... 104
5.1.2 Troubles in BF Microscopy ........................................................................................... 107
5.1.3 Troubles in Ph Microscopy ............................................................................................ 109
5.1.4 Troubles in DIC and IMSI Microscopies ....................................................................... 110
5.1.5 Troubles in Epi-FL Microscopy ..................................................................................... 111
5.1.6 Troubles in DF Microscopy ........................................................................................... 111
5.1.7 Troubles in NAMC Microscopy ..................................................................................... 112
5.2 Troubleshooting for the Units ..................................................................................................... 113
5.2.1 Microscope Main Body ................................................................................................. 113
5.2.2 Problems with the Dia-illumination Section .................................................................. 113
5.2.3 Pillar for Dia Illumination and Condenser ..................................................................... 114
5.2.4 Stage ............................................................................................................................. 114
5.2.5 Nosepiece and Focusing Device .................................................................................. 114
5.2.6 Epi-fluorescence Attachment ........................................................................................ 114
Chapter 6 Maintenance and Storage of the Device ................................................................................ 115
6.1 Cleaning the Device ................................................................................................................... 116
6.1.1 Cleaning the Lenses ..................................................................................................... 117
6.1.2 Cleaning Parts other than Lenses ................................................................................ 117