OPTIMedical OPTI CCA-TS - Page 181

Operator’s Manual – OPTI CCA-TS pH-3
ANALYTES pH
Linearity
4
Wherever possible, linearity for the OPTI CCA measurement has been established against reference
materials or methods. Linearity for pH of whole blood is established by measurement of blood specimens
which were tonometered to various CO
2
values, and measured on an AVL 995 pH/Blood Gas Analyzer
standardized to N.I.S.T. traceable pH buffers, and on three OPTI CCA-TS systems.
Correlation
 Slope Intercept Coefcient Sy.x  Range  n
1.0174 -0.1099 0.99972 0.006 6.85 – 7.67 81
Correlation to Other Methods
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OPTICCAvsotherpH/BloodGasInstrumentsonwholebloodinatypicalsetting
Excess blood aliquots from specimens collected for blood gas analyses were analyzed by both traditional
and non-traditional operators of blood gas equipment in hospital laboratories. The blood was analyzed on
the OPTI CCA after obtaining the requisite results from existing instrumentation used for these analyses
and operated and controlled following their established procedures.
Correlation
ComparativeMethod* Slope Intercept Coefcient Sy.x Range n
Analyzer A (whole blood) 0.9269 0.534 0.9789 0.013 7.19 – 7.56 103
Analyzer B (whole blood) 1.0800 -0.579 0.9954 0.009 7.01 – 7.55 173
Analyzer C (whole blood) 1.126 ± 0.018 -0.946 ± 0.134 0.9868 0.018 7.09 – 7.58 105
Analyzer D (whole blood) 1.003 ± 0.008 -0.032 ± 0.058 0.9947 0.014 6.86 – 7.63 174
Analyzer E (whole blood) 1.104 ± 0.010 -0.739 ± 0.077 0.9919 0.014 6.80 – 7.60 183
* For more information on specic analyzers used, please contact OPTI Medical Technical Support.
References
1. Tietz, Norbert W., Ed., Clinical Guide to Laboratory Tests, 2nd Ed., (Philadelphia: W.B.Saunders,
Co., 1990) p. 436
.
2. Peterson JI, et.al., A Fiber Optic pH Probe for Physiological Use, Anal.Chem. 53,p.864, 1980.
3. Wolfbeis OS, Offenbacher H, Fluorescence Sensor for Monitoring Ionic Strength and Physiological
pH Values, Sensors and Actuators 9, p.85, 1986.
4. OPTI Medical. Model equation for regression statistics is: [results of OPTI Analyzer] = slope(m)
[comparative method results] + intercept(b).