OPTIMedical OPTI CCA-TS - Page 216

Cl-2 Operator’s Manual – OPTI CCA-TS
CHLORIDE ANALYTES
Interferences
The OPTI CCA-TS Cl
-
sensor does exhibit a signicant (greater than 2:1) positive interference from
bromide, iodide, interlipid and nitrite. Minor interference is observed from phenylacetic acid salicyate and
thiocynate.
Only clear, uncolored quality control materials, such as OPTI CHECK or OPTI CHECK PLUS brand
aqueous controls should be used with the OPTI CCA-TS system. Colored materials, including prociency
testing materials, may interfere with the ion measurement, or fail to be properly aspirated
.
Heparin salts are the only acceptable anticoagulants. Other anticoagulants such as citrate, EDTA, oxalate,
and uoride cause signicant interferences to the electrolyte sensors.
Reproducibility
Typical Within-Run (Swr), Between-Day (Sdd) and Total (ST) Precision is determined from 1 run per
day with 2 replicates per run for 20 days on each of two OPTI CCA instruments. Chloride values are
expressed in mmol/L.
Material mean Swr (CV%) Sdd (CV%) ST (CV%)
OPTI CHECK PLUS
(aqueous control solution)
Level 1 76.8 0.12 (0.2) 0.51 (0.7) 0.53 (0.7)
Level 2 100.2 0.10 (0.1) 0.74 (0.7) 0.75 (0.8)
Level 3 116.8 0.11 (0.1) 0.45 (0.4) 0.47 (0.4)
Serum 97.6 0.06 (0.1) 1.03 (1.1) 1.03 (1.1)
Reduced Bovine Hemoglobin 79.2 0.07 (0.1) 0.78 (1.0) 0.80 (1.0)
Solution
All specic performance characteristics tests were run with default instrument calibration and after
normal recommended equipment quality control checks were performed (see Operator’s Manual).
Specimens at each level were analyzed in replicates of two for 20 days. The within-run and
between-day standard deviations were calculated by the analysis of variance method.
Linearity
3
Wherever possible, linearity for the OPTI CCA measurement has been established against reference
materials or methods. Chloride linearity is established by measurement of gravimetrically prepared,
N.I.S.T. traceable aqueous standard solutions (Chloride
ST
). Chloride linearity in serum is established
against Chloridometry (Chloride
CL
)
Correlation
 Slope Intercept Coefcient Sy.x Range n
Chloride
ST
1.0076 -0.56 0.99984 0.68 58 - 160 15
Chloride
CL
1.0064 -2.44 0.99823 1.66 74 - 142 16