110 BioPAT
®
 Trace | Multi Trace Operating Instructions
 Operation
Status Action LED1 
(green)
LED 2 
color
LED 2 
status
Calibrations
Calibration loaded on blue on
Calibration unloaded on blue flashes
Reference loaded on blue on
Reference unloaded on blue flashes
Malfunctions
No network 
connection
on red on
Initialisation error on black off
8.5  Tips
8.5.1  Choosing Suitable Standard Solutions
Various combinations of calibration standard solutions are available to 
optimally cover the expected concentration cycles. Sartorius recommends 
connecting a higher concentrated standard than standard1. 
Depending on the process conditions and the objective of the cultivation, 
various concentration ranges have to be covered. The concentration range 
should principally be between the concentrations of the standard solutions in 
order to ensure optimal measuring accuracy. Furthermore, we recommend 
always choosing a standard with very low concentration in order to properly 
determine measured values close to zero.
For regulation, the concentrations of the calibration standards should not be 
too far from the regulation (set)point. For regulation at 1g/L, for example, it is 
more practical to use a combination of 0.5g/L and 4g/L than a combination 
of 1g/L and 10g/L, for example.