82 BioPAT
®
Trace | Multi Trace Operating Instructions
Operation
NOTICE
Make sure that the three-way stopcock is set as
displayed. Otherwise all following calibrations will
be erroneous and the resulting measurements
incorrect!
Before you continue working, make sure that the
three-way valve was set back to the initial
position after removing the external sample, so
that you can correctly measure Standard 1 during
the next calibration.
8.2.1 Fast Reference (Only for Dialysis)
In use of the dialysis method it is necessary to
perform a referencing in order to match the per-
meability of the membranes within the reactor
and outside in the diffusion cell, and to compen-
sate for temperature differences between the
two membranes. A reliable determination of the
reference factor is therefore very important for
the quality of the measurement results.
Normally, a measurement is triggered by the
“Reference” option and the measurement result
is communicated. The user can than confirm the
measured values (reference factor does not
change), correct the value or perform the analy-
sis of an external sample. For processes with slow
concentration changes (e.g. cell cultures) and
already known concentration values in the
medium, this procedure can be accelerated by
using the “Fast Reference”.
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