Sartorius BioPAT Trace - Cleaning the Dialysis Probe and Replacing the Membrane

62 BioPAT
®
Trace | Multi Trace Operating Instructions
Initial Installation
As a general rule, air bubbles in the tubing set are flushed out over time.
However, this process takes a certain amount of time (e.g. during the priming
process). If there are air bubbles in the measuring cell after connecting the
dialysis probe, then proceed as follows:
t Operate the system for a period of 30 minutes in measurement mode with
maximum measuring frequency.
6.3 Cleaning the Dialysis Probe and Replacing
theMembrane
After harvesting the bioreactor or any dead autoclaving phase, proceed
asfollows:
t Loosen the tube ends and disassemble the tubing set from the probe.
t Remove the dialysis probe from the bioreactor.
t Use a soft brush and water to clean the surface of the probe and
cover caps.
t Check the gaskets of the dialysis probe adapter for cleanliness and ensure
that they are seated correctly.
t Unscrew the two TORX screws from the lower part of the dialysis probe.
t Disassemble the dialysis probe lunette with the membrane from the lower
part of the dialysis probe.
t Clean the dialysis probe lunette and the lunette holder with a soft brush
and water.
NOTICE
If the residues are not removed, the dialysis probe may become obstructed
and leakages may occur in the tubing set.
t Remove the residue from inside the dialysis probe.
t Flush the inside of the dialysis probe with deionized water. Use the
single-use syringe for this.
t Air dry the inside of the dialysis probe. Use the single-use syringe
for this.