Sartorius BioPAT Trace - Single Sample; Reference (for Dialysis Only)

BioPAT
®
Trace | Multi Trace Operating Instructions 79
Operation
8.1.4 Single Sample
This function can be used for a spontaneous unscheduled single point mea-
surement at a selected port (Multi Trace). This function is especially useful
during long intervals, when for instance an actual value of a certain reactor is
wanted. By this option, the running program does not need to be interrupted
and the planned intervals remain unchanged.
The “Single Sample” command is only triggered, if there is enough available
time for the measurement.
8.2 Reference (for Dialysis only)
A reference measurement must be performed to compare the permeability of
the two diusion membranes in the diusion cell (which is only allowed
contact with calibration solutions) and in the dialysis probe (which only has
contact with the reactor contents). The relationship of the two membranes to
one another is expressed by a reference factor used for calculating the
measured values.
You can conduct a reference measurement whenever you want, in particular
when changes occur in the process that may influence the diusion speed
(e.g. as a result of temperature changes).
Reference measurements can also be carried out at regular intervals, e.g.
when samples are taken for external accompanying analysis. The measured
value is only corrected with a new reference factor when the new reference
value was entered and conrmed by pressing “Apply”. Up to then, measured
values that were determined in the meantime are always calculated and
displayed with the currently valid reference factor.
The device starts with a reference factor of 1.0. If the two membranes were
permeable to the same degree, then this value would be correct. In practice,
however, experience has shown that manufacturing-related and ambient
conditions aecting the probe can lead to discrepancies.