Sartorius BioPAT Trace - Diffusion Module Function

BioPAT
®
Trace | Multi Trace Operating Instructions 35
Design and Function
4.5 Diffusion Module Function
The diffusion module is part of the tubing set. The standard solutions or an
external sample are pumped through the diffusion module to the analysis
system. A membrane enables diffusion of the substrate from the donor
solutions (both standard and sample) into the buffer solution (acceptor). The
different membrane materials enable dialysis, cellulase-stable dialysis and gas
diffusion for separating substrates. The following figure shows a schematic
diagram of this system:
Donor
Acceptor
Membrane
Fig. 11: “Schematic diagram of the diffusion module system”
The module is made of PMMA. It has a diffusion area of 20 mm
2
and ensures
that a sufficient exchange of substances takes place between the donor and
the acceptor stream. The donor and acceptor streams flow in through the
lower module inlet. The drain is located at the top.
t In order to ensure reliable operation of the measuring instrument, the
diffusion module must be secured in the correct position on the retaining
plate (arrow pointing up).