18 BioPAT
®
Trace | Multi Trace Operating Instructions
BioPAT
®
Trace | Multi Trace Product Description
Conditioning of the sample is based on the method of flow diffusion analysis
(Figure 4). This method works with a time-dependent sample transfer. For
that purpose, the measurement line and the sample and |or the sample
infeed are separated from each other by a membrane. The measuring
distance is called the acceptor (receiving) and the sample side is called the
donor (giving).
During an analysis, the measurement line is flushed with a transport buffer,
which is an acceptor stream that can be stopped from behind the membrane
for a defined period of time. This procedure is carried out by triggering a valve,
which in turn opens up a bypass route.
Meanwhile, the analytes in the sample diffuse through the membrane and, at
the same time, are enriched in the holding chamber. In this way, a continually
increasing concentration of dialyzable components develops in the fluid
segment of the transport buffer.
Membrane
Transport buffer
Holding chamber
Outlet
Step 1: Rinsing
Step 2: Stop flow and analyte enrichment
Step 3: Transporting the accumulated material to the conductivity cell
Fig. 4: Principle of the flow diffusion technique
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