40 BioPAT
®
Trace | Multi Trace Operating Instructions
Initial Installation
6.1.1.1 Preparing the Transport Buffer
The transport buffer is a salt mixture (for cell cultures) or a concentrate (for
microbial cultures) and must be dissolved or diluted with water (deionized or
distilled) according to the instructions. Proper dilution can be checked by
measuring the conductivity of an aliquot. The diluted buffer must have a
conductivity of 5 – 15mS/cm.
To avoid contamination in the tubing set, it is recommended that the diluted
transport buffer be sterile filtered and/or placed in sterile vessels such as
bottles or bags. Safety measures to avoid contamination in the tubing set are
summarized in Chapter “8.5.4 Avoiding Contaminations in the Tubing Set”,
page 112.
CAUTION
Erroneous measurements due to incorrect or no dilution!
The transport solution is delivered as a salt mixture or concentrate. Observe
the note for proper resolution/dilution on the packaging.
The consumption of transport buffer depends on the measuring interval and
the number of measuring points when it is in operation. For a typical single-
channel measurement in dialysis mode, the following consumption can
beexpected:
Dialysis mode
Interval Buffer consumption 10 L for:
min µl/min ml/h ml/d h d
1 835.6 50.1 1,203.2 199 8
5 835.6 50.1 1,203.2 199 8
10 614.4 36.9 884.7 271 11
20 356.3 21.4 513.1 468 19
30 270.3 16.2 389.3 617 26
60 184.3 11.1 265.4 904 38
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