Thermo NanoDrop One User Manual

Measure Kinetics
156 NanoDrop One User Guide Thermo Scientific
 To measure a sample using the Kinetics application
1. From the instrument Home screen, select the Kinetics tab and tap the Kinetics icon.
The Kinetics Setup screen is displayed. If one or more kinetic methods exist in the
currently selected Data Storage Location, they will be listed in the Select Method box. A
description of the selected method appears in the Method Details box.
2. Select a method:
• select an existing method by tapping the method name in the Select Method box
• create a new method by tapping Create Method, specifying the method settings
and choosing Save Method
• edit an existing method by tapping the method name and choosing Edit Method
3. Specify any cuvette options such as heating or stirring by tapping > Settings (see
General settings for details).
Note: If your cuvette pathlength is not 10 mm, specify the correct pathlength in
General Settings.
4. Tap Run Method.
5. Measure a blank:
– Fill clean, dry cuvette with enough blanking solution to cover instrument optical
path
– Lift instrument arm and insert blanking cuvette into cuvette holder, making sure to
align light path of cuvette with light path of instrument
–Tap Blank

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)