Thermo NanoDrop One User Manual

3
Learning Center
Instrument Settings
238 NanoDrop One User Guide Thermo Scientific
Dye/Chrom. Editor
Use the Dye/Chromophore Editor to add a custom dye to the list of available dyes in
Microarray Setup or Proteins & Labels Setup. You can also specify which dyes are available in
that list.
Protein Editor
Use the Protein Editor to add a custom protein to the list of available proteins in the
Protein A280 application.
Related Topics
• Set Up the Instrument
• Update Software
• Measure a Sample Using a Cuvette
• Set Up Ethernet Connection
• Dye/Chromophore Editor
• Protein Editor
Use cuvette Select cuvette sampling mode (available for NanoDrop One
C
instrument model only). When selected, these additional
options are available:
Pathlength: Enter cuvette pathlength (width) before taking
blank or sample measurements with cuvettes (see cuvette
manufacturer for cuvette specifications)
Stir Speed: If using automatic stirring, drop micro-stir bead
into sample cuvette and set Stir Speed (levels 1 through 9
correspond with range from 10 RPM to 850 RPM with
controlled ramping from zero)
Heat cuvette to 37 °C: Select this option if sample cuvettes
require heating. Cuvette heater increases from room
temperature to 37 °C at rate of 5 °C/minute.

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)