Measure Proteins and Labels
Thermo Scientific NanoDrop One User Guide 71
To measure a labeled protein sample
1. From the Home screen, select the Proteins tab and
then tap Protein & Labels.
2. Specify the sample type and the type of dye(s) used.
Tip: Select a dye from the pre-defined list or add a
custom dye using the Dye/Chromophore Editor.
3. Pipette 1–2 μL of the blanking solution onto the
lower pedestal and lower the arm, or insert the
blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to align the cuvette
light path with the instrument light path.
4. Tap Blank and wait for the measurement to
complete.
Tip: If Auto-Blank is On, the blank measurement
starts automatically after you lower the arm. (This
option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new
laboratory wipe, or remove the blanking cuvette.
6. Pipette 2 μL sample solution onto the pedestal and
lower the arm, or insert the sample cuvette into the
cuvette holder.
7. Start the sample measurement:
–Pedestal: If Auto-Measure is On, lower arm; if
Auto-Measure is off, lower arm and tap Measure.
– Cuvette: Tap Measure
When the sample measurement is completed, the
spectrum and reported values are displayed (see the
next section).
8. When you are finished measuring samples, tap
End Experiment.
9. Lift the arm and clean both pedestals with a new
wipe, or remove the sample cuvette.
Dye absorbance peak
used to calculate dye
concentration
A280 absorbance peak
used to calculate protein
concentration
Peptide
backbone
Typical sample spectrum measured with
Proteins & Labels application
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