OPERATION
Carl Zeiss Illumination and contrast methods Axio Imager
154 430000-7344-001 M70-2-0020 e 06/2009
The DIC method employs polarized light and therefore is disturbed by birefringent objects, such
as films, in the light path between polarizer and analyzer, as are occasionally used in
conjunction with histological sections. The same applies to acrylic glass culture chambers, if the
chamber bottom is made of plastic. In these cases, it is advisable to use chambers with bottom
plates of glass to avoid loss of optical performance.
1 DIC slider
2 Knurled screw
3 Reflector turret
4 Objective on nosepiece
5 Sliding button for aperture diaphragm
6 Condenser with DIC prism and polarizer
7 Compensator λ
8 Analyzer module
Fig. 4-120 Components required for transmitted-light DIC
To Next Page
Loading...
