Columns and Traps Part 4. Conditioning columns
Procedure: Conditioning a capillary column
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Procedure: Conditioning a capillary column
WARNING Do not use hydrogen as the carrier for conditioning! It could vent into the oven
and present an explosion hazard.
1. Select an appropriate column pressure—given as psi (kPa)—from this table.
2. Enter the selected pressure. Let gas flow through the column at room
temperature for 15 to 30 minutes to remove air.
3. Program the oven temperature from room temperature to the maximum
temperature for the column. Increase the temperature at a rate of
10 to 15°C/min and hold at the maximum temperature for 30 minutes.
4. If you will not be using the conditioned column immediately, remove it from
the oven. Cap both ends to prevent air, moisture, and other contaminants
from entering the column.
Inside diameter
Length, m 0.10 mm 0.20 mm 0.25 mm 0.32 mm 0.53 mm
10 25 (170) 6 (40) 3.7 (26) 2.3 (16) 0.9 (6.4)
15 39 (270) 9 (61) 5.6 (39) 3.4 (24) 1.4 (9.7)
25 68 (470) 15 (104) 9.5 (65) 5.7 (40) 2.3 (16)
30 83 (570) 18 (126) 12 (80) 7 (48) 2.8 (19)
50 32 (220) 20 (135) 12 (81) 4.7 (32)
60 39 (267) 24 (164) 14 (98) 5.6 (39)
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