44 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
3 Good Measurement Practices
General Considerations
Lens tissues for glasses or other uses often contain detergents or
lubricants which can affect your measurements. If possible avoid cleaning
the faces of your cell between blank and sample measurements.
Handling Cells
Always install a cell so that it faces the same direction to minimize
problems with cell non- uniformity. For best results with microcuvettes,
leave your sample cell clamped in position throughout the measurement
sequence. Solutions should be removed and replaced by pipette or use
flow cells.
Figure 10 Spectrum taken with floating particles in the light path
200
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1000
-0.05
-0.04
-0.03
-0.02
-0.01
0.00
0.01
Wavelength [nm]
Absorbance
If glass pasteur pipettes are used, make sure that the optical windows of the cell are
not touched or scratched by the pipette.
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