Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 43
Good Measurement Practices 3
General Considerations
Handling and Maintaining Cells or Cuvettes
Passivating New Cells
When filling a non- passivated new cell with your sample, you will observe
that air bubbles stick on the windows of your cell. To prevent the
formation of sticky bubbles, rinse the cell with cleaning and passivating
fluid (part number 5062- 8529). The cleaning procedure is described on the
label of the cleaning fluid container.
Cleaning Cells
The fats in fingerprints are significant absorbers in the UV region and, if
left on optical surfaces, can cause erroneous results. Wipe off all
fingerprints and contaminants before using a sample cell.
Use only high quality lens tissues (part number 9300- 0761) and never
dry the inside of a cell with lens tissues. Dry the inside of the cell with
pressurized, oil free air, that prevents the cell from contamination by
tissue particles, or rinse the cell with blank or sample solution. Floating
particles in the cell will deflect the light beam and so lead to a very poor
quality of the measured spectrum.
Figure 9 Floating particles in a cell
floating particles will deflect and
scatter the light beam
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