Agilent Technologies Cary 8454 - Page 49

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 49
Good Measurement Practices 3
General Considerations
Figure 11 Example of a blank on water showing a good baseline
Figure 12 Example of a blank on water with bubbles causing a poor baseline
200
400
600
800
1000
-0.0010
-0.0005
0.0000
0.0005
0.0010
0.0015
200
400
600
800
1000
Wavelength [nm]
Absorbance
200
400
600
800
1000
-0.05
-0.04
-0.03
-0.02
-0.01
0.00
0.01
Wavelength [nm]
Absorbance
NOTE
If your blank or spectra show artifacts similar to the one in Figure 12, see “Solvents” on
page 45 to optimize the measurement procedure.