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Agilent Technologies Cary 8454
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62 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
4 Installing Accessories
Optical Filter Wheel
The choice of when to use a filter and which filter to use should be made
only after considering all the factors that affect your measurements. If you
decide that a filter is needed, you should choose a filter that transmits the
wavelengths needed for analytical information and that blocks the
wavelengths that contribute to sample degradation. Often, your final
choice will be a compromise since sometimes even the light at the
wavelengths of analytical interest can affect your sample.
If you do not need a filter for a group of measurements, you can move the
filter wheel to position zero. This permits full light transmission. When
you will not be using the filter wheel for an extended period of time you
should remove the filter wheel assembly from the cell holder and store it
in a clean plastic bag.
Figure 21 Transmission characteristics of filter wheel filters
Table 3 Filter wheel positions
Position Filter
0 None
1 UV roll-off
2 265 nm UV cut-off (at 50%T)
3 295 nm UV cut-off (at 50%T)
190 200 300 400 500 600 700 800 820
0
20
40
60
80
100
0
1
2
3

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