BIO RAD CFX96 User Manual

Gene Expression Analysis
120
The standard error for the normalization factor in the normalized expression formula is shown
here:
Where:
• n = Number of reference targets
• SE = Standard error
• NF = Normalized expression
• RQ = Relative quantity
The standard error for normalized gene of interest (GOI) formula is shown here:
Where:
• SE = Standard error
• GOI = Gene of interest (one target)
• NF = Normalization factor
• n = Number of reference targets
SE NF
n
NF
n
SE RQ
sample (Ref 1)
nSE RQ
sample (Ref 1)
×
------------------------------------------------------
⎝⎠
⎛⎞
2
SE RQ
sample (Ref 2)
nSE RQ
sample (Ref 2)
×
------------------------------------------------------
⎝⎠
⎛⎞
2
…
SE RQ
sample (Ref n)
nSE RQ
sample (Ref n)
×
------------------------------------------------------
⎝⎠
⎛⎞
2
+++×=
SE GOI
n
GOI
n
SE NF
n
NF
n
------------------
⎝⎠
⎛⎞
2
SE GOI
GOI
-------------------
⎝⎠
⎛⎞
+
2
×=

BIO RAD CFX96 Specifications

General

Temperature Uniformity	±0.4 °C
Software	CFX Maestro Software
Detection Method	Real-time PCR (qPCR)
Sample Capacity	96-well plate
Excitation Source	LED
Ramp Rate	Up to 5°C/sec
Heating Rate	5°C/sec
Sensitivity	Single copy detection
Power Requirements	100-240 V, 50/60 Hz
Temperature Range	0–100 °C