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Eppendorf Xplorer - 5.9.2 Aspirating liquid; 5.9.3 Dispensing liquid

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34
Operation
Eppendorf Xplorer
®
/Eppendorf Xplorer
®
plus
English (EN)
5.9.2 Aspirating liquid
5.9.3 Dispensing liquid
1. Wet the new pipette tip by aspirating and dispensing the liquid to be
pipetted one to three times.
2. Vertically immerse the pipette tip into the liquid approx. 4 mm during
aspiration.
3. Press the rocker up to aspirate liquid. Maintain the immersion depth to
ensure no air is accidentally aspirated.
4. For large volumes, keep the pipette tips in the liquid for approx. 3 seconds
after aspiration.
5. After aspiration, slowly pull the pipette tip out of the liquid.
6. Wipe the pipette tip slowly against the tube wall to ensure that no outer
wetting remains on the pipette tip.
1. When dispensing liquid, place the pipette tip at a slight angle against the
wall of the tube.
2. Press the rocker down to dispense the liquid.
3. After dispensing, wait until no more liquid is dispensed.
4. Press the rocker down again to trigger the blow-out (Blow).
Stopping the piston movement
Pressing the rocker in the other direction when the piston is still moving will stop
the piston movement. You can then move the piston either up or down by
pressing the rocker in the corresponding direction. Pressing the rocker up will
abort a mixing process.
Operating modes with the rocker pressed down: in the Man mode, the ongoing
piston movement is immediately stopped by releasing the rocker. In the Ads
mode, an ongoing dispensing step is completed after the rocker is released. The
next dispensing step is only performed when the rocker is pressed accordingly.

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