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Operation
Eppendorf Xplorer
®
/Eppendorf Xplorer
®
plus
English (EN)
5.12.2 Reverse pipetting (Xplorer)
By using the blow-out, more liquid is aspirated with reverse pipetting than with standard
pipetting. During dispensing, the blow-out (Blow) does not belong to the dispensing
volume. Reverse pipetting is advantageous for plasma, serum and other liquids with a
high protein content. Reverse pipetting is not necessary for pipetting aqueous solutions.
Prerequisites
• The pipette is switched on.
• The Pipetting (Pip) operating mode has been selected.
• The parameters have been set.
• The pipette tip has been attached.
1. Keep the rocker pressed down.
2. Immerse the pipette tip in the liquid.
3. Release the rocker to aspirate the blow-out.
4. Press the rocker up to aspirate the dispensing volume.
5. Place the pipette tip on the wall of the target tube.
6. Press the rocker down to dispense the liquid.
7. Remove the pipette tip from the destination tube. Residual liquid remains in the tip.
There are two courses of further action:
Aspirate the same liquid from the same reservoir tube.
Press the rocker up to aspirate the same liquid. The residual liquid in the tip is used for
further processing.
Dispense a different liquid
1. Press the rocker up to discard the residual liquid in the tip.
2. Press the ejector to eject the pipette tip.
Use a new pipette tip for the next dispensing operation.
1 Keep the rocker pressed
2 Aspirate the blow-out
3 Aspirate
4 Dispense
5 Aspirate or empty
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