Eppendorf Xplorer - 5.15.4 Reverse pipetting of liquid - Spc selection dial setting

Operation
Eppendorf Xplorer
®
/Eppendorf Xplorer
®
plus
English (EN)
47
5.15.3.1 Example
Required auxiliary aids:
• Xplorer plus with a nominal volume of 300 µL
• 4 dispensing tubes (200 µL)
5.15.4 Reverse pipetting of liquid – Spc selection dial setting
The Rev. Pipetting mode is well suited for plasma, serums and other liquids with a high
protein content. The Pipetting mode is also well suited for aqueous solutions. The Rev.
Pipetting mode is also well suited for solutions containing wetting agents in order to
minimize the formation of foam during dispensing in the target tube. The liquid is
aspirated using blow-out (Blow). The blow-out is not part of the dispensing volume and
may not be dispensed into the target tube. If you use the same liquid again, the blow-out
may remain in the tip. If you use a different liquid, discard the blow-out and the pipette
tip.
Prerequisites
• The pipette is switched on.
• The Rev. Pipetting (Spc) operating mode has been selected.
• The parameters have been set.
• The pipette tip has been attached.
Tube 1 Tube 2 Tube 3 Tube 4
Volume/step
30 µL 60 µL 90 µL 120 µL
Volume/step
120 µL 90 µL 60 µL 30 µL
Dilution 1+X 1+4 1+1.5 1+0.67 1+0.25
Dilution 1:Y 1:5 1:2.5 1:1.67 1:1.25
Tube total 150 µL 150 µL 150 µL 150 µL
1 Aspiration
2 Dispensing
3 Aspirate or empty
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