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20 Appendices
20.1 Reagent Consumption
Function
Reagent consumption / function (ml)
Erba Lyse-5P Erba Diff-5P Erba Dil-Diff
Start Up 13.6 1.7 128
Measure Blank 5.7 0.7 52
Measure 5 part 5.7 0.7 52
Measure Calibration 5.7 0.7 52
Measure QC 5.7 0.7 52
Measure SSM blank 5.7 0.7 60
Measure SSM 5-part 5.7 0.7 60
Measure SSM Calibration 5.7 0.7 60
Measure SSM QC 5.7 0.7 60
Standby 0 0 11.2
Wakeup 0 0 10
Standby in SSM 0 0 11.2
Wakeup in SSM 0 0 11
Cleaning 6.7 0.7 133
Hard cleaning 6.7 0.7 144.2
Clean in SSM 6.7 0.7 138
Hard clean in SSM 6.7 0.7 154
Flow cell cleaning 0 0 63
Shear Valve cleaning 9 1.2 100.2
Offline (overnight) rinsing 6.7 0.7 72.2
**Prime Diluent (full) 0 0 103
**Prime Lyse 7.5 0 2.5
**Prime Diff5P 0 4 0
**Prime all 22 11.5 103
*DrainDilu 0 0 120
*DrainLyse 60 0 0
*DrainDiff5P 0 60 0
*DrainAll 60 60 180
FillUp 22 12 105
Shutdown 7.7 0.7 153.2
* If internal chambers are filled (volume of chambers: 50 ml each, diluent 2x50 + 50 sheath)
** Prime functions fill internal chambers. The following pneumatic function uses reagents from the inside puffer while the
inside puffer is continuously refilled to a steady level.
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