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Figure 7. Optical Signal Processing System
External structure (and the size of the cell) causes lower angles of scatter, and internal
granularity or complexity causes higher angles of diffraction. Both low and high angles of
light scatter are captured by optical sensors, providing the ‘ELite 5’ analyzer with two
independent measurements for each cell crossing the path of the laser beam.
Figure 8. Cellular Light Scatter
The light scatter data are gathered and plotted as a two dimensional scatter diagram. Similar
cells have similar scatter characteristics and tend to group together. This allows the
analytical software to differentiate and identify the leukocyte populations and generate the
4DIFF and BASO scatter diagrams.
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