Troubleshooting
5
5-2 Gilson 157/159 UV-VIS Detectors User’s Guide
Troubleshooting
Troubleshooting
When troubleshooting a problem, first do the following:
1 Check all cabling.
2 Check all fittings.
3 Check for air in the tubing.
4Check for leaks.
5 Look for system messages on the display.
Device Will Not Turn On
Inspect the power cable to be sure it is plugged into the power supply.
Device Cannot Be Calibrated
• Tighten the knurled-head screws on the cover plate to prevent incursion from interfering light or an
electronic error.
•Insert the test cell.
• Inspect the calibration with a weak absorbing eluent.
Relationship of the Signal to the Light Path Reference is Low
•Purge the flowcell.
• Clean the flow cell window.
• Exchange lamp (spectrum source).
Baseline Noise
• Tighten the knurled-head screws on the cover plate to prevent incursion from interfering light or an
electronic error.
• Allow the lamp to warm up for at least 15–30 minutes.
• Eliminate drafty air currents and variations in ambient temperature. Move the detector away from windows
or doors.
• Check grounds on all instruments. Eliminate ground loops by connecting the detector and recorder to the
same power outlet.
• Be sure solvents are degassed.
• If you see baseline deflection when back pressure is exerted on the flow cell, you probably have bubbles
trapped in the cell. Try one of these methods to eliminate bubbles:
• Increase back pressure on the cell by connecting pressure regulator tubing to the flow cell outlet.
• Increase back pressure by raising flow rate and using a syringe or septum to momentarily block exit
stream.
•Flush flowcell using a syringe filled with fresh solvent.
• Raise the fraction collector to a level higher than the flow cell.
• The lamp intensity may be weak. Refer to Lamp Replacement
on page 4-9.
• Check flow cell for contamination. Refer to Flow Cell Maintenance
on page 4-2.
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