Gilson 157/159 UV-VIS Detectors User’s Guide 5-3
Troubleshooting
5
Troubleshooting
Baseline Drift
• Maintain constant temperature conditions during the measurement.
• Allow the lamp to warm up for at least 15–30 minutes.
• Eliminate changes in ambient temperature. If drift is dependent on temperature, eliminate drafts and avoid
areas of direct sunlight.
• Check for contaminated or “bleeding” column. If drift disappears after removing column connections,
regenerate or replace column.
• Be sure solvents are degassed.
• If you see baseline deflection when back pressure is exerted on the flow cell, you probably have bubbles
trapped in the cell. Try one of these methods to eliminate bubbles:
• Increase back pressure on the cell by connecting pressure regulator tubing to the flow cell outlet.
• Increase back pressure by raising flow rate and using a syringe or septum to momentarily block exit
stream.
• Flush flow cell using a syringe filled with fresh solvent.
• Raise the fraction collector to a level higher than the flow cell.
• Check pump or delivery system to determine that flow rate is constant.
• The lamp intensity may be weak. Refer to Lamp Replacement
on page 4-9.
• Check flow cell for contamination. Refer to Flow Cell Maintenance
on page 4-2.
LAN (Ethernet) Connection
For communication to occur, the instrument must be connected via a LAN (Ethernet) connection to a router. If
there are communication problems between the instrument and the computer, verify that the procedure below
has been followed.
1 Connect the router to a power source and then wait 30 seconds for the router to initialize.
2 With the instrument powered OFF, connect one end of an Ethernet cable to a port on the router and the other
end to the ETHERNET port on the rear panel of the instrument.
3 Power the instrument ON and then wait 1 minute for it to initialize. The instrument will be assigned an IP
address via DHCP from the router at this time.
4 Using an Ethernet cable, connect one end of the cable to the connector on the computer and the other end to
a port on the router.
Confirm Communication
After the connections have been verified, use the procedure below to confirm communication.
1 Reboot the computer.
2 Turn off power to the router and the detector.
3 Wait five minutes.
4 Turn power on to the router.
5 Turn power on to the detector.
6 Start TRILUTION® LC Software.
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