Gilson 157 - Efficient HPLC Separations; Avoid Additional Dead Volume; Use Special Columns; Use Filtered Solvents

Gilson 157/159 UV-VIS Detectors User’s Guide 5-5
Troubleshooting 5
Efficient HPLC Separations
Efficient HPLC Separations
To make your HPLC separations as efficient as possible, pay close attention to the following:
Avoid Additional Dead Volume
1 Once they have been used, never re-use tubing in other areas of the HPLC system.
2 Only use a given PEEK fitting for one specific port and never re-use it for other ports. Always install new PEEK
fittings on each separate port.
Use Special Columns
When using special columns, follow the manufacturer’s instructions on caring for the columns!
Use Filtered Solvents
1 Use ultra-pure, filtered solvents for HPLC – gradient grade.
2 Filtration of substances under analysis.
3 Use of inline filters.
Check for Clogged Tubing
Regularly check for clogged tubing. Test back pressure without a column.