Chapter 5 Troubleshooting
105
Problem Check item
The image quality is poor.
Specimen / stage
Check the orientation of the cover glass surface of the specimen. (
☞
3.10.1)
Check if the cover glass thickness is appropriate. (
☞
3.10.1)
If uneven blurring occurs, or an out-of-focus condition occurs as a result of stage
movement, check whether the specimen is tilted.
Nosepiece / objective
Check that there is no air bubbles between the specimen and the objective. (Oil
immersion objective:
☞
3.10.2)
Remove the DIC slider from the optical path. (
☞
3.9.2)
Correctly adjust the correction collar of the objective. (
☞
3.10.1)
Use appropriate immersion liquid. (
☞
1.2.7, 3.10.2, 3.10.3)
Clean the tip of the objective. (
☞
6.1.1)
Condenser
Adjust the height of the condenser. (
☞
3.4.2)
Filters
Remove the GIF filter if the field of view is green. (
☞
3.3.4)
If the field of view has coloring, remove the filter cube or the barrier filter from the
optical path. (
☞
3.12.1)
Environment
Check if there is ambient light (such as display screen light) entering the image.
(
☞
3.3.6)
Correctly select the optical path and eliminate the influence from the ambient light by
the split optical path. (
☞
3.2.1)
Dirt or dust is highly visible in
the
image
.
Maintenance
Clean the surface of specimens and culture vessels
Clean the objective. (
☞
6.1.1)
Clean the eyepieces. (
☞
6.1.1)
Clean the condenser. (
☞
6.1.1)
If dirt or dust moves when the camera is turned, clean the camera.
Clean the relay lens. (
☞
6.1.1)
Check the upper surface of the condenser lens and the lower surface of the
objective.
The image is dark.
Polarizer / Analyzer
Remove the polarizer from the optical path. (
☞
3.5.1)
Pull out the analyzer slider until it clicks. (
☞
3.5.2)
Remove the analyzer block from the optical path (
☞
3.5.2)
FL turret
Remove the filter cube from the optical path. Place the required filter cube into the
optical path. (
☞
3.12.1)
Tube base unit
Correctly set the optical path changeover knob. (Eyepiece tube base unit with port:
☞
3.7.1)
The image is large.
Intermediate magnification dial
Return the intermediate magnification dial to 1x. (
☞
3.2.2)
Camera
Confirm the adapter's variable magnification lens setting. (
☞
7.14)
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