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PP Systems CIRAS-2 User Manual

PP Systems CIRAS-2
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Glossary of Fluorescence Terms
CIRAS-2 Operator's Manual Version 2.04
- 125 -
Glossary of Fluorescence Terms
Definitions of the most commonly referenced fluorescence parameters are given below with alternative
nomenclatures.
Parameter
Alternative
Name
Definition
Measurement Method
Fo
(Fluorescence origin)
F nought
F zero
O
(in Kautsky
terms)
Signal following dark adaptation
when all PSII reaction centres and
electron acceptors are fully
oxidised, hence “open” for
photochemistry.
Modulated System - monitor
signal under modulating
beam only from dark
adapted sample.
Continuous Excitation -
monitor induction with high
time resolution (10 µs).
Must have high time
resolution.
Fp
(Fluorescence
peak)
P
Peak signal following illumination
with non-saturating pulse of light.
Not generally used today as most
modern instruments have saturating
light sources which can deliver
>2,500 µmolm
-2
s
-1
.
Track fluorescence rise and
determine peak fluorescence
value.
Fm
(Fluorescence
maximum)
Fmax
(P in Kautsky
terms)
Maximum fluorescence signal from
the dark-adapted leaf following
application of a saturating light
pulse which fully reduced the PSII
electron acceptors preventing
photochemistry.
Dark adapt sample, apply
saturating light pulse and
determine maximum
fluorescence signal.
Fv
(Variable
Fluorescence)
Variable
fluorescence
Derived from Fm-Fo
Automatically calculated
Fv/Fm
PSII
MAX
Derived from (Fm-Fo)/Fm.
Directly proportional to the
maximum quantum efficiency of
PSII.
Automatically calculated
Area over OIDP curve.
Area
Proportional to the size of the PSII
electron transport acceptor pool.
Automatically calculated
Fs
(Steady state
fluorescence)
Ft
(terminal T in
Kautsky terms)
Signal under an actinic light.
Switch on actinic lamp on
modulated system.
Sometimes used to show
“terminal” fluorescence
signal when photosynthesis
is activated at the end of a
Kautsky curve.
Fm‟
(light-adapted
fluorescence maximum)
Fms
Maximum fluorescence signal from
light-adapted leaf following
application of saturating pulse
which fully reduced PSII electron
acceptors preventing
photochemistry.
Adapt sample to actinic light,
deliver saturating pulse and
track fluorescence signal.

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PP Systems CIRAS-2 Specifications

General IconGeneral
BrandPP Systems
ModelCIRAS-2
CategoryMeasuring Instruments
LanguageEnglish

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