Recording Chlorophyll Fluorescence
CIRAS-2 Operator's Manual Version 2.04
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Each chlorophyll fluorescence calculation is time stamped on the left. To view each individual
measurement, simply click on the time stamp of interest and the chlorophyll fluorescence data associated
with that point in time is displayed.
After performing an Fv/Fm calculation following the dark adaptation period, there will normally be a
“recovery” period (in seconds) set by the user under CFM Recording Options to allow the sample to
recover from the dark adaptation period. You should notice a stable fluorescence signal at approximately
700 units. When the photosynthesis readings are stable, press the thumb record button on the leaf
cuvette or click Single in the Recording window to record a measurement.
In addition to measurement of photosynthesis, a phiPSII is calculated followed by a “recovery period” (in
seconds) set by the user under CFM Recording Options to allow the sample to recover from the light
adapted measurement. To perform a phiPSR at the conclusion of all measurements (example below),
click on the PSR button in the upper left hand corner of the display.
Response Curve Recording with CFM
Click on Recording - Begin and select „Response Curve‟. This will bring up the Response Curve Editor.
Full information on setting up Response Curves can be found at Response Curve on page 68.
Click on Recording Options and make your selections (example as shown below):
Click on CFM Options. They should be exactly the same as set up earlier in this manual. If you would
like to change any of the CFM options, you can do so here. When your Response Curve-Control Settings
are set, click on OK to return to the Response Curve Editor. Any change here will also change the
settings in the CFM file.
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