Recording Chlorophyll Fluorescence
CIRAS-2 Operator's Manual Version 2.04
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Recording Chlorophyll Fluorescence
As described in the main CIRAS-2 Operation Manual, measurements are initiated by selecting Recording
- Begin. There are 4 types of recording that can be achieved with CIRAS-2:
Key Press
Timed
Response Curve (manual or automatic)
Batch Response Curve
CIRAS-2 with CFM is designed for simultaneous measurement of gas exchange and chlorophyll
fluorescence. Except for the intial Fv/Fm, every measurement begins with a photosynthesis measurement
followed by a chlorophyll fluorescence measurement based on the CFM settings described earlier.
The following sections describe the two most common types of measurements, Keypress and Response
Curve Recording.
Keypress Recording with CFM
Click on Recording - Begin and select „Keypress‟.
The measurement will begin based on the adaptation type selected earlier followed by
photosynthesis/chlorophyll fluorescence measurements based on the cuvette environment (Parameters
Editor) settings (i.e. CO
2
, H
2
O, Light, etc.) and CFM recording options. If you need to change any of the
CFM recording options, click on Keypress Options and then Edit CFM options.
In this case, we made the following CFM selections based on Dark Adaptation. After a 30 minute dark
adaptation period, the CIRAS-2 will perform an Fv/Fm calculation based on the CFM settings below:
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